Primmorphs (a three-dimensional sponge primary cell culture system) have been revealed to be a cell/tissue nano-factory for the production of tailor-made hybrid nanostructures. Growth of primmorphs is stimulated by the presence of a titanium alkoxide precursor tolerating titania (TiO(2)) concentrations up to 250 mu M. The presence and activity of silicatein in primmorphs has been analyzed by gel electrophoresis and Western blotting. Results of studies by scanning transmission electron microscopy with energy-dispersive X-ray spectroscopy have revealed silica and titania to be co-localized on nanosized spicules. Our findings suggest that the incorporation of titania into the nanosized spicule is enzymatically mediated via active silicatein in an orchestrated mechanism. RI Kolb, Ute/A-2642-2011; tahir, Muhammad Nawaz/B-7650-2011; Natalio, Filipe/D-4417-2011; Tremel, Wolfgang/D-8125-2011; Mugnaioli, Enrico/E-6237-2011
Silicatein-mediated incorporation of titanium into spicules from the demosponge Suberites domuncula
Mugnaioli E;
2010-01-01
Abstract
Primmorphs (a three-dimensional sponge primary cell culture system) have been revealed to be a cell/tissue nano-factory for the production of tailor-made hybrid nanostructures. Growth of primmorphs is stimulated by the presence of a titanium alkoxide precursor tolerating titania (TiO(2)) concentrations up to 250 mu M. The presence and activity of silicatein in primmorphs has been analyzed by gel electrophoresis and Western blotting. Results of studies by scanning transmission electron microscopy with energy-dispersive X-ray spectroscopy have revealed silica and titania to be co-localized on nanosized spicules. Our findings suggest that the incorporation of titania into the nanosized spicule is enzymatically mediated via active silicatein in an orchestrated mechanism. RI Kolb, Ute/A-2642-2011; tahir, Muhammad Nawaz/B-7650-2011; Natalio, Filipe/D-4417-2011; Tremel, Wolfgang/D-8125-2011; Mugnaioli, Enrico/E-6237-2011I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
