Measles is one of the most infectious airborne viruses worldwide. With a basic reproduction rate between 12–18, this virus is six times more infectious than the SARS-CoV-2 Alpha variant and similar to the SARS-CoV-2 Omicron variant. Even though a cheap and effective vaccine is available, measles is still common in developing countries. To date, sporadic outbreaks are also reported in developed countries, primarily due to non-vaccinated people. This work presents a point-of-care (POC) biosensing device capable of detecting measles virions (MV) in human saliva. The device is a surface-acoustic-wave (SAW) based lab-on-a-chip (LOC), smaller than a €1-cent coin, in which SAWs are used both for sensing and fluid recirculation. The biosensing detection performance of this system is tested and device sensitivity and selectivity are assessed. The SAW-LOC with MV loaded in healthy, whole human saliva is finally validated. The experimental results also highlight a crucial aspect of the biosensing process: the interactions between probing and target species during incubation with or without fluid mixing. The presented findings are promising for realizing a POC platform for measles diagnosis and may serve as a guideline for designing new microfluidics-based biosensing systems.

Surface-Acoustic-Wave (SAW) Induced Mixing Enhances the Detection of Viruses: Application to Measles Sensing in Whole Human Saliva with a SAW Lab-On-a-Chip

Lunardelli F.;Luminare A. G.;Lai M.;Pistello M.;
2022-01-01

Abstract

Measles is one of the most infectious airborne viruses worldwide. With a basic reproduction rate between 12–18, this virus is six times more infectious than the SARS-CoV-2 Alpha variant and similar to the SARS-CoV-2 Omicron variant. Even though a cheap and effective vaccine is available, measles is still common in developing countries. To date, sporadic outbreaks are also reported in developed countries, primarily due to non-vaccinated people. This work presents a point-of-care (POC) biosensing device capable of detecting measles virions (MV) in human saliva. The device is a surface-acoustic-wave (SAW) based lab-on-a-chip (LOC), smaller than a €1-cent coin, in which SAWs are used both for sensing and fluid recirculation. The biosensing detection performance of this system is tested and device sensitivity and selectivity are assessed. The SAW-LOC with MV loaded in healthy, whole human saliva is finally validated. The experimental results also highlight a crucial aspect of the biosensing process: the interactions between probing and target species during incubation with or without fluid mixing. The presented findings are promising for realizing a POC platform for measles diagnosis and may serve as a guideline for designing new microfluidics-based biosensing systems.
2022
Agostini, M.; Lunardelli, F.; Gagliardi, M.; Miranda, A.; Lamanna, L.; Luminare, A. G.; Gambineri, F.; Lai, M.; Pistello, M.; Cecchini, M.
File in questo prodotto:
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11568/1149588
 Attenzione

Attenzione! I dati visualizzati non sono stati sottoposti a validazione da parte dell'ateneo

Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 25
  • ???jsp.display-item.citation.isi??? ND
social impact