Cytophotometric and biochemical analyses indicate changes with plant development in the amount and organization of the nuclear DNA in epidermal and subepidermal tissues excised from different portions of tobacco stems: DNA is progressively lost with tissue ageing, while amplification of repeated sequences occurs in tissues developed after phase change. These genome variations are related to the morphogenetic response of the tissues cultured in vitro, which differs both quantitatively and qualitatively: loss of DNA sequences progressively impairs morphogenetic capability; DNA amplification may be a factor of the explant's ability to regenerate flowers. These results point to the possible role of the nuclear DNA condition of differentiated tissues in determining their morphogenetic expression in vitro.

NUCLEAR-DNA CHANGES DURING PLANT DEVELOPMENT AND THE MORPHOGENETIC RESPONSE INVITRO OF NICOTIANA-TABACUM TISSUES

CAVALLINI, ANDREA;CREMONINI, ROBERTO;
1987

Abstract

Cytophotometric and biochemical analyses indicate changes with plant development in the amount and organization of the nuclear DNA in epidermal and subepidermal tissues excised from different portions of tobacco stems: DNA is progressively lost with tissue ageing, while amplification of repeated sequences occurs in tissues developed after phase change. These genome variations are related to the morphogenetic response of the tissues cultured in vitro, which differs both quantitatively and qualitatively: loss of DNA sequences progressively impairs morphogenetic capability; DNA amplification may be a factor of the explant's ability to regenerate flowers. These results point to the possible role of the nuclear DNA condition of differentiated tissues in determining their morphogenetic expression in vitro.
Altamura, Mm; Bassi, P; Cavallini, Andrea; Cionini, G; Cremonini, Roberto; Monacelli, B; Pasqua, G; Sassoli, O; Van, Ktt; Cionini, Pg
File in questo prodotto:
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11568/11538
 Attenzione

Attenzione! I dati visualizzati non sono stati sottoposti a validazione da parte dell'ateneo

Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 24
  • ???jsp.display-item.citation.isi??? 29
social impact