There are very few previous reports of expression of native full-length maedi visna virus (MVV) Env gp150 protein in the literature. Therefore the use of different plasmid and viral expression vectors to obtain full-length gp 150 was investigated. A mammalian expression plasmid, pN3-Env, was constructed containing the MVV env gene encoding the precursor protein gp150 Env. The functionality of the recombinant plasmid was tested for expression in HEK293 cells. A recombinant modified vaccinia Ankara virus, MVA-Env, with expression detected in avian cells was also made. The expression of the MVV gp 150 Env precursor protein was shown for the first time upon transfection of the eukaryotic HEK293 cells by the pN3-Env plasmid DNA as demonstrated by Western blot analysis. These plasmid or viral expression vectors are of potential use in MVV vaccines. (c) 2007 Elsevier B.V. All rights reserved.

Expression of the gp150 maedi visna virus envelope precursor protein by mammalian expression vectors

TOLARI, FRANCESCO;
2007

Abstract

There are very few previous reports of expression of native full-length maedi visna virus (MVV) Env gp150 protein in the literature. Therefore the use of different plasmid and viral expression vectors to obtain full-length gp 150 was investigated. A mammalian expression plasmid, pN3-Env, was constructed containing the MVV env gene encoding the precursor protein gp150 Env. The functionality of the recombinant plasmid was tested for expression in HEK293 cells. A recombinant modified vaccinia Ankara virus, MVA-Env, with expression detected in avian cells was also made. The expression of the MVV gp 150 Env precursor protein was shown for the first time upon transfection of the eukaryotic HEK293 cells by the pN3-Env plasmid DNA as demonstrated by Western blot analysis. These plasmid or viral expression vectors are of potential use in MVV vaccines. (c) 2007 Elsevier B.V. All rights reserved.
Fraisier, C; Arnarson, H; Barbezange, C; Andresdottir, V; Carrozza, Ml; De Andres, D; Tolari, Francesco; Rosati, S; Lujan, L; Pepin, M; Amorena, B; Harkiss, G; Blacklaws, B; Suzan Monti, M.
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11568/116097
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