Two new metabolites, (4S)-(+)-ascochin (1a) and (S,S)-(+)-ascodiketone (3), together with the known compounds (3R,4R)-(–)-4-hydroxymellein (2), ent-α-cyperone (4) and (3S,4R)-(–)-dihydroxy-(6S)-undecyl-α-pyranone (5) were isolated from cultures of the of the endophytic fungus Ascochyta sp. The biologically active isocoumarin derivative, (4S)-(+)-ascochin (1a), has an unusual substitution pattern which was confirmed by X-ray diffraction. Its absolute configuration was determined by our solid-state TDDFT CD methodology using the X-ray coordinates as input for the calculation. By catalytic hydrogenation, (4S)-(+)-ascochin was converted into the corresponding (3S,4S)-dihydroisocoumarin derivative 1b. The measured and TDDFT calculated CD spectra enabled studies on the correlation between absolute configuration and n-π* transition Cotton effect.
|Autori:||KROHN K; KOCK I; ELSSSER B; FLOERKE U; SCHULZ B; DRAEGER S; PESCITELLI G; ANTUS S; KURTAN T|
|Titolo:||Bioactive Natural Products from the Endophytic Fungus Ascochyta sp. From Meliotus dentatus – Configurational Assignment by Solid-State CD and TDDFT Calculations|
|Anno del prodotto:||2007|
|Digital Object Identifier (DOI):||10.1002/ejoc.200600907|
|Appare nelle tipologie:||1.1 Articolo in rivista|