We study here a DNA oligonucleotide having the abi l i t y to form two different i-motif structures whose rela t i v e stabi l i t y depends on pH and temperature. The major species at neutral pH is stabilized by two C:C+ base pairs capped by two minor groove G:C:G:C tetrads. The high pH and thermal stabi l i t y of this structure are ma i n l y due to the favorable effect of the minor groove tetrads on their adjacent positively charged C:C+ base pairs. At pH 5, we observe a more elongated i-motif structure consisting of four C:C+ base pairs capped by two G:T:G:T tetrads. Molecula r dynamics calculations show that the conformational transition between the two structures is driven by the protonation state of key cytosines. In spite of large conformational differences, the transition between the acidic and neutral structures can occur without unfolding of the i-moti f . These results represent the first case of a conformational switch between two different i-motif structures and illustrate the dramatic pH-dependent plasticity of this fascinating DNA motif .

pH-Dependent Capping Interactions Induce Large-Scale Structural Transitions in i-Motifs

Cupellini, Lorenzo;
2023-01-01

Abstract

We study here a DNA oligonucleotide having the abi l i t y to form two different i-motif structures whose rela t i v e stabi l i t y depends on pH and temperature. The major species at neutral pH is stabilized by two C:C+ base pairs capped by two minor groove G:C:G:C tetrads. The high pH and thermal stabi l i t y of this structure are ma i n l y due to the favorable effect of the minor groove tetrads on their adjacent positively charged C:C+ base pairs. At pH 5, we observe a more elongated i-motif structure consisting of four C:C+ base pairs capped by two G:T:G:T tetrads. Molecula r dynamics calculations show that the conformational transition between the two structures is driven by the protonation state of key cytosines. In spite of large conformational differences, the transition between the acidic and neutral structures can occur without unfolding of the i-moti f . These results represent the first case of a conformational switch between two different i-motif structures and illustrate the dramatic pH-dependent plasticity of this fascinating DNA motif .
2023
Serrano-Chacón, Israel; Mir, Bartomeu; Cupellini, Lorenzo; Colizzi, Francesco; Orozco, Modesto; Escaja, Núria; González, Carlos
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11568/1171205
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