Natriuretic peptides (NPs) and endothelin (ET)-1 system appear to be associated with the development and progression of many cardiovascular disease (CD) linked to obesity. Aim of this study was to evaluate their transcriptional alterations by Real Time-PCR in cardiac tissues of Zucker rats subdivided in obese rats (O, n=20), and controls (CO, n=20): half under fasting conditions (COfc-Ofc) and the remainders after the induction of acute hyperglycemia (COAH-OAH). Thyroid function was also determined using MagPix system.Lower BNP and ANP mRNA were observed in O with respect to CO while CNP expression resulted higher probably for its endothelial role. A down-regulation of NPR-B and NPR-C and an upregulation of NPR-A was observed in O. Lower pre-proET-1 and ECE-1 mRNA levels were observed in O with respect to CO (p=0.02). ET-A and ET-B mRNA showed no significant differences between CO and O. Significantly higher plasma levels of T3 (p=0.01), T4 (p=0.02) and lower levels of TSH (p=0.005) in O with respect to CO, were found. The more relevant pathway observed subdividing into fasting and hyperglycemic rats are reported in Fig1.The results of this study suggest a reduced release of NPs by cardiac cells as well as of its counter-regulatory ET-1 system and could be a useful starting point for future studies aimed to identify new therapeutic strategies for the treatment of cardiometabolic syndrome.
Obesity modulates the transcriptional alterations of the natriuretic peptide and endothelin systems
Belcastro, E;
2013-01-01
Abstract
Natriuretic peptides (NPs) and endothelin (ET)-1 system appear to be associated with the development and progression of many cardiovascular disease (CD) linked to obesity. Aim of this study was to evaluate their transcriptional alterations by Real Time-PCR in cardiac tissues of Zucker rats subdivided in obese rats (O, n=20), and controls (CO, n=20): half under fasting conditions (COfc-Ofc) and the remainders after the induction of acute hyperglycemia (COAH-OAH). Thyroid function was also determined using MagPix system.Lower BNP and ANP mRNA were observed in O with respect to CO while CNP expression resulted higher probably for its endothelial role. A down-regulation of NPR-B and NPR-C and an upregulation of NPR-A was observed in O. Lower pre-proET-1 and ECE-1 mRNA levels were observed in O with respect to CO (p=0.02). ET-A and ET-B mRNA showed no significant differences between CO and O. Significantly higher plasma levels of T3 (p=0.01), T4 (p=0.02) and lower levels of TSH (p=0.005) in O with respect to CO, were found. The more relevant pathway observed subdividing into fasting and hyperglycemic rats are reported in Fig1.The results of this study suggest a reduced release of NPs by cardiac cells as well as of its counter-regulatory ET-1 system and could be a useful starting point for future studies aimed to identify new therapeutic strategies for the treatment of cardiometabolic syndrome.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
