Xenon and other inhalational agents induce cell and organ protection through different and only partially elucidated molecular mechanisms. Anesthesia induced or pharmacologic preconditioning is a recognized mechanism of cell protection. In this study we explored the gene transcription of activity-dependent neuroprotective protein (ADNP) in neonatal rat brain as consequence to xenon exposure, comparing the noble gas to nitrogen. Seven-day-old Sprague Dawley rats were exposed for 120 min to 75% xenon and 25% oxygen or control condition consisting of 75% nitrogen and 25% oxygen (Air). ADNP was found to be differentially expressed by SSH, validated by Relative Real-Time PCR (RT-PCR) and confirmed by western blot and immunohistochemistry. The differential expression of ADNP in the rat neonatal brain may account for the preconditioning and neuroprotective effects exerted by gas xenon.
|Autori:||CATTANO D; VALLEGGI S; MA D; KASTSIUCHENKA O; ABRAMO A; SUN P; CAVAZZANA AO; NATALE G; GIUNTA F|
|Titolo:||Xenon induces transcription of ADNP in neonatal rat brain|
|Anno del prodotto:||2008|
|Appare nelle tipologie:||1.1 Articolo in rivista|