Vascular calcification is a systemic disease characterized by calcium salt deposition within vascular walls. Here, we present a protocol for establishing an advanced dynamic in vitro co-culture system using endothelial and smooth muscle cells to replicate vascular tissue complexity. We describe steps for cell culture and seeding in a double-flow bioreactor that recreates the action of blood in humans. We then detail the induction of calcification, setting up of the bioreactor, followed by cell viability assessment and calcium quantification.

Protocol to generate an in vitro model to study vascular calcification using human endothelial and smooth muscle cells

Cecchettini A;
2023-01-01

Abstract

Vascular calcification is a systemic disease characterized by calcium salt deposition within vascular walls. Here, we present a protocol for establishing an advanced dynamic in vitro co-culture system using endothelial and smooth muscle cells to replicate vascular tissue complexity. We describe steps for cell culture and seeding in a double-flow bioreactor that recreates the action of blood in humans. We then detail the induction of calcification, setting up of the bioreactor, followed by cell viability assessment and calcium quantification.
2023
Persiani, E; Ceccherini, E; Gisone, I; Cecchettini, A; Vozzi, F
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11568/1186968
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