We performed full modeling analysis of 5-HT1A-[F-18] MPPF interactions using the beta-microprobe (beta P) and a YAP-(S) PET scanner. Sixteen Wistar rats were used for beta P (n= 5) and YAP-(S) PET (n= 5) acquisitions and metabolite studies (n= 6). Time -concentration curves were obtained in the hippocampus, raphe dorsalis, frontal cortex and cerebellum, using three injections of [ 18F] MPPF at different specific activities. B'(max) values were estimated from a two (2T-5k)-and three (3T-7k)-tissue-compartment model with beta P and YAP-(S) PET time concentration curves. The simplified reference tissue model (SRTM) was used to estimate binding potential (BPSRTM) values from data obtained with the first injection and the cerebellum as the reference region. Overall, the 3T-7k model provided a better fit than the 2T-5k model, as evaluated from AIC criteria in all experiments. The rank order of receptor density (B'(max)) values was as follows: hippocampus > raphe approximate to frontal cortex > cerebellum. Non-negligible specific binding was observed in the cerebellum (B'(max) (beta P)= 1.5 +/- 0.9 pmol/ ml). Significant correlations (pb0.001) between B'(max) and BPSRTM values were evident with both beta P (r= 0.895) and YAP-(S) PET (r= 0.695). The YAP-(S) PET system underestimated the [ 18F] MPPF binding levels in brain due to limited resolution (i. e. partial volume), but led to similar conclusions. (C) 2008 Elsevier Inc. All rights reserved.

In vivo quantification of 5-HT1A-[F-18] MPPF interactions in rats using the YAP-(S) PET scanner and a beta-microprobe

DEL GUERRA, ALBERTO;
2008

Abstract

We performed full modeling analysis of 5-HT1A-[F-18] MPPF interactions using the beta-microprobe (beta P) and a YAP-(S) PET scanner. Sixteen Wistar rats were used for beta P (n= 5) and YAP-(S) PET (n= 5) acquisitions and metabolite studies (n= 6). Time -concentration curves were obtained in the hippocampus, raphe dorsalis, frontal cortex and cerebellum, using three injections of [ 18F] MPPF at different specific activities. B'(max) values were estimated from a two (2T-5k)-and three (3T-7k)-tissue-compartment model with beta P and YAP-(S) PET time concentration curves. The simplified reference tissue model (SRTM) was used to estimate binding potential (BPSRTM) values from data obtained with the first injection and the cerebellum as the reference region. Overall, the 3T-7k model provided a better fit than the 2T-5k model, as evaluated from AIC criteria in all experiments. The rank order of receptor density (B'(max)) values was as follows: hippocampus > raphe approximate to frontal cortex > cerebellum. Non-negligible specific binding was observed in the cerebellum (B'(max) (beta P)= 1.5 +/- 0.9 pmol/ ml). Significant correlations (pb0.001) between B'(max) and BPSRTM values were evident with both beta P (r= 0.895) and YAP-(S) PET (r= 0.695). The YAP-(S) PET system underestimated the [ 18F] MPPF binding levels in brain due to limited resolution (i. e. partial volume), but led to similar conclusions. (C) 2008 Elsevier Inc. All rights reserved.
Millet, P; Moulin, M; Bartoli, A; DEL GUERRA, Alberto; Ginovart, N; Lemoucheux, L; Buono, S; Fagret, D; Charnay, Y; Ibanez, V.
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11568/119847
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