LisosanG is a fermented flour that contains polyphenols, polyunsaturated fatty acids, vitamins, and alpha-lipoic acid with antioxidant/anti-inflammatory properties. These compounds are often characterized by instability, low solubility/bioavailability, and poor absorption that restrict their application in therapy. These problems can be solved by using delivery systems among which liposomes have emerged as prominent candidates. Liposomes can load compounds with varied physico-chemical properties, providing protection from degradation, increased solubility, modulation of release, and facilitated diffusion across biomembranes. Lisosan G liposomes tailored for oral administration were prepared. Eudragit (R) enteric polymer was added to protect the liposomes from acidic pH. The Eudragit-liposomes were around 100 nm and unilamellar. They were resistant to acidic conditions and did not alter cell viability, nor intracellular ROS levels. These findings confirm that liposomes are an efficient system for the loading of multicomponent-based extracts and that, when properly formulated, can offer protection from physiological degradation, and be safely applied to cells.

Tackling the challenges of the oral administration of a fermented flour extract with liposomes: Effect on intestinal HT-29 cells

Tomassi, Elena
Co-primo
;
Lucchesi, Daniela
Co-primo
;
Sancho, Veronica;
2023-01-01

Abstract

LisosanG is a fermented flour that contains polyphenols, polyunsaturated fatty acids, vitamins, and alpha-lipoic acid with antioxidant/anti-inflammatory properties. These compounds are often characterized by instability, low solubility/bioavailability, and poor absorption that restrict their application in therapy. These problems can be solved by using delivery systems among which liposomes have emerged as prominent candidates. Liposomes can load compounds with varied physico-chemical properties, providing protection from degradation, increased solubility, modulation of release, and facilitated diffusion across biomembranes. Lisosan G liposomes tailored for oral administration were prepared. Eudragit (R) enteric polymer was added to protect the liposomes from acidic pH. The Eudragit-liposomes were around 100 nm and unilamellar. They were resistant to acidic conditions and did not alter cell viability, nor intracellular ROS levels. These findings confirm that liposomes are an efficient system for the loading of multicomponent-based extracts and that, when properly formulated, can offer protection from physiological degradation, and be safely applied to cells.
2023
Tomassi, Elena; Lucchesi, Daniela; Caddeo, Carla; Valenti, Donatella; Sancho, Veronica; Pons, Ramon; Pucci, Laura
File in questo prodotto:
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11568/1220348
 Attenzione

Attenzione! I dati visualizzati non sono stati sottoposti a validazione da parte dell'ateneo

Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 0
  • ???jsp.display-item.citation.isi??? 0
social impact