The flavour and aroma of certain Vitis vinifera grape varieties is dominated by volatile terpenes and small volatile aldehydes. Monoterpenes contribute to the final grape and wine aroma and flavour in form of free volatiles and as glycoside conjugates of monoterpene alcohols. Typical monoterpenol components of the cultivar Muscat and other aroma-rich grape varieties are linalool, geraniol, nerol, citronellol, and alpha-terpineol. These compounds are widely recognized by humans as important fragrance, flavour and aroma compounds and can present desirable quality traits for plant breeding in viticulture. The aim of this project is the molecular characterization of genes of terpenoid formation in Muscat, an aromatic Vitis vinifera varieties. In this work, we describe the expression of different monoterpene and sesquiterpene synthases, and other genes involved flavour biosynthesis pathway in flower buds, open flowers, and during berry ripening and we compare transcriptional profiles with aroma profiles obtained by solid phase extraction analysis. The experiments reveal that monoterpenes synthases are almost synthesized during berry setting and the sesquiterpenes synthases are almost synthesized in flower buds, open flowers, during berry setting and in harvest stage. The aroma profiles show almost terpenoids production in flower buds and in harvest stage.
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