Owing to the increasing development of nanotechnology, there is a need to assess how engineered nanomaterials can interact with living cells. The main purpose of the present study was to assess whether metal cobalt nanoparticles (CoNP 100–500 nm) are genotoxic compared to cobalt ions (Co21). Uptake experiments were carried out by incubating peripheral blood leukocytes (PBLs) with 57Co21 (added to stable Co21 1022 M to obtain concentrations in the range of 1025 to 1024 M) or with 60CoNP for 24 and 48 h. Whereas intracellular Co21 showed slight or no variations over the baseline levels, CoNP were taken up efficiently leading to intracellular CoNP concentrations of 485 6 106.1 and 970 6 99 fg per cell after 24 and 48 h, respectively. The genotoxicity end points considered in this study were the frequency of binucleated micronucleated (BNMN) cells and the percentage of tail DNA (% Tail DNA) fragmentation by means of the comet assay. Genotoxic effects were evaluated by incubating PBLs of three healthy donors with subtoxic concentrations (1025 to 8 3 1025M) of Co21 in the form of cobalt chloride, CoNP and ‘washed’ CoNP, the latter to exclude any interference by Co21. On a group basis, Co21 induced a clear trend in the increase of the BNMN frequency, whereas CoNP showed only minor changes. Moreover, we observed a high variability among donors in the induction of micronuclei. The comet assay showed a statistically significant doserelated increase in % Tail DNA for CoNP (P < 0,001), whereas Co21 did not induce significant changes over control values. These findings suggest that nanosized Co can be internalized by human leukocytes and can interact with DNA leading to the observed genotoxic effects, which are, however, modulated both by donor’s characteristics and/or by Co21 release.
Comparative genotoxicity of cobalt nanoparticles and ions on human peripheral leukocytes in vitro
MIGLIORE, LUCIA
2008-01-01
Abstract
Owing to the increasing development of nanotechnology, there is a need to assess how engineered nanomaterials can interact with living cells. The main purpose of the present study was to assess whether metal cobalt nanoparticles (CoNP 100–500 nm) are genotoxic compared to cobalt ions (Co21). Uptake experiments were carried out by incubating peripheral blood leukocytes (PBLs) with 57Co21 (added to stable Co21 1022 M to obtain concentrations in the range of 1025 to 1024 M) or with 60CoNP for 24 and 48 h. Whereas intracellular Co21 showed slight or no variations over the baseline levels, CoNP were taken up efficiently leading to intracellular CoNP concentrations of 485 6 106.1 and 970 6 99 fg per cell after 24 and 48 h, respectively. The genotoxicity end points considered in this study were the frequency of binucleated micronucleated (BNMN) cells and the percentage of tail DNA (% Tail DNA) fragmentation by means of the comet assay. Genotoxic effects were evaluated by incubating PBLs of three healthy donors with subtoxic concentrations (1025 to 8 3 1025M) of Co21 in the form of cobalt chloride, CoNP and ‘washed’ CoNP, the latter to exclude any interference by Co21. On a group basis, Co21 induced a clear trend in the increase of the BNMN frequency, whereas CoNP showed only minor changes. Moreover, we observed a high variability among donors in the induction of micronuclei. The comet assay showed a statistically significant doserelated increase in % Tail DNA for CoNP (P < 0,001), whereas Co21 did not induce significant changes over control values. These findings suggest that nanosized Co can be internalized by human leukocytes and can interact with DNA leading to the observed genotoxic effects, which are, however, modulated both by donor’s characteristics and/or by Co21 release.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
