Among flavonoids, chalcones have been identified as interesting compounds having antioxidant, antimutagenic and antitumour properties. In this study we have evaluated the clastogenicity of plicatin B on human lymphocytes (whole blood and isolated lymphocytes) and its anticlastogenic activity, in the same cellular systems, using mitomycin C (MMC), radio-mimetic bleomycin (BL) and hydrogen peroxide (H2O2) as reference clastogens. Plicatin B per se resulted cytotoxic at high dose but non-clastogenic. The clastogenic effects induced by MMC and BL were significantly reduced (-33%) whereas with H2O2 the protective effect of plicatin B was observed (-62%) only at the greater H2O2 dose. The anticlastogen effects of plicatin B were effective when the chalcone was introduced in the culture, 1 h before the reference clastogens.
Protective effects of plicatin B on micronucleus induction in cultured human lymphocytes by different mutagens
SCARPATO, ROBERTO;
2009-01-01
Abstract
Among flavonoids, chalcones have been identified as interesting compounds having antioxidant, antimutagenic and antitumour properties. In this study we have evaluated the clastogenicity of plicatin B on human lymphocytes (whole blood and isolated lymphocytes) and its anticlastogenic activity, in the same cellular systems, using mitomycin C (MMC), radio-mimetic bleomycin (BL) and hydrogen peroxide (H2O2) as reference clastogens. Plicatin B per se resulted cytotoxic at high dose but non-clastogenic. The clastogenic effects induced by MMC and BL were significantly reduced (-33%) whereas with H2O2 the protective effect of plicatin B was observed (-62%) only at the greater H2O2 dose. The anticlastogen effects of plicatin B were effective when the chalcone was introduced in the culture, 1 h before the reference clastogens.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
