In this study, the chemical content of the threatened Cretan endemic Chaerophyllum creticum Boiss. & Heldr. was investigated by High Performance Liquid chromatography-Photodiode Array-Mass Spectrometry (HPLC-PDA-MS) analysis. Leaves, flowers and stems of C. creticum, were extracted via maceration and were assessed for their polyphenolic composition and antioxidant capacity. The highest extraction yields were achieved by methanol and methanol/water. A total of 17 compounds were characterised in C. creticum with luteolin-7-O-glucoside being the predominant glucoside found in all the extracts. Malonic esters were present in all the extracts. The main flavonoids and phenolics were quantified by HPLC-UV in parallel to standard spectrophotometric assays which were used for the determination of the Total Polyphenol content and the Total Flavonoid Content. The antioxidant activity was assessed by two different tests: 2,2-diphenyl-1-picrylhydrazyl (DPPH•) free radical assay and the ferric reducing antioxidant power (FRAP) assay. This is the first report on the chemical content of the Cretan endemic C. creticum.
Threatened Cretan species Chaerophyllum creticum Boiss. & Heldr.: phenolic profile by HPLC-PDA-MS and in vitro antioxidant capacity
Karioti A.
2022-01-01
Abstract
In this study, the chemical content of the threatened Cretan endemic Chaerophyllum creticum Boiss. & Heldr. was investigated by High Performance Liquid chromatography-Photodiode Array-Mass Spectrometry (HPLC-PDA-MS) analysis. Leaves, flowers and stems of C. creticum, were extracted via maceration and were assessed for their polyphenolic composition and antioxidant capacity. The highest extraction yields were achieved by methanol and methanol/water. A total of 17 compounds were characterised in C. creticum with luteolin-7-O-glucoside being the predominant glucoside found in all the extracts. Malonic esters were present in all the extracts. The main flavonoids and phenolics were quantified by HPLC-UV in parallel to standard spectrophotometric assays which were used for the determination of the Total Polyphenol content and the Total Flavonoid Content. The antioxidant activity was assessed by two different tests: 2,2-diphenyl-1-picrylhydrazyl (DPPH•) free radical assay and the ferric reducing antioxidant power (FRAP) assay. This is the first report on the chemical content of the Cretan endemic C. creticum.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
