Incubation of leech segmental ganglia with [(32)P]phosphoric acid results in the labeling of 30 phosphoproteins, detected by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, followed by autoradiography. Treatment with 8-bromo-cAMP or phosphodiesterase inhibitors induces a rise in the label of the following phosphoproteins: 96, 79, 63, 50, 40, 32, 28, 24, 23 and 20 kDa. One of the most intersting membrane-bound proteins studied has an apparent molecular weight of 79 kDa. It seems to be specific for the nervous tissue and inside the nervous system, for segmental ganglia. The role of these phosphoproteins in the mechanisms of learning processes is discussed.
cAMP-dependent protein phosphorylation of a 79kDa protein in segmental ganglia of the leech Hirudo medicinalis
GARCIA GIL, MARIA de las MERCEDES;
1989-01-01
Abstract
Incubation of leech segmental ganglia with [(32)P]phosphoric acid results in the labeling of 30 phosphoproteins, detected by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, followed by autoradiography. Treatment with 8-bromo-cAMP or phosphodiesterase inhibitors induces a rise in the label of the following phosphoproteins: 96, 79, 63, 50, 40, 32, 28, 24, 23 and 20 kDa. One of the most intersting membrane-bound proteins studied has an apparent molecular weight of 79 kDa. It seems to be specific for the nervous tissue and inside the nervous system, for segmental ganglia. The role of these phosphoproteins in the mechanisms of learning processes is discussed.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
