Comet assay is a quick and versatile technique for assessing DNA damage in individual cells. It allows the detection of DNA single- and double-strand breaks, as well as the presence of alkali-labile sites and cross-links. Here we describe protocols for the Single-Cell Gel Electrophoresis (Comet assay) in its alkaline (pH > 13), mild alkaline (pH = 12.1), and neutral (pH = 8) versions when applied in aquatic animals exposed to nanomaterials. © The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2026.
The Comet Assay in Nano-ecogenotoxicology
Patrizia GuidiWriting – Review & Editing
;Giada Frenzilli
Writing – Original Draft Preparation
2026-01-01
Abstract
Comet assay is a quick and versatile technique for assessing DNA damage in individual cells. It allows the detection of DNA single- and double-strand breaks, as well as the presence of alkali-labile sites and cross-links. Here we describe protocols for the Single-Cell Gel Electrophoresis (Comet assay) in its alkaline (pH > 13), mild alkaline (pH = 12.1), and neutral (pH = 8) versions when applied in aquatic animals exposed to nanomaterials. © The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2026.File in questo prodotto:
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