In this study we analysed the polymorphisms of retrotransposon based molecular markers (IRAR REMAR SSAP) in 22 Vitis genotypes: 16 Vitis vinifera genotypes (eight different 'Sangiovese' clones, four different 'Niellucciu' clones, 'Prugnolo gentile', 'Ciliegiolo', 'Trebbiano toscano', 'Vermentino') Vitis vinifera subsp. sylvestris, Vitis rupestis du Lot, Vitis cinerea, Vitis cordifolia, Vitis champinii, and Vitis rotundifolia. In particular, we studied the polymorphisms of three different retrotransposons found in the grapevine genome, two copia-like, Vine-I and, Tvv1, and one gypsy-like, Gret1. Among these, Tvv1 showed the highest polymorphisms and allowed us to clearly distinguish among Vitis species and Vitis vinifera varieties analysed. On the same genotypes, we also analysed some SSR, I-SSR, and AFLP polymorphisms. The phylogenetic tree obtained from the Tvv1 polymorphisms and those obtained from I-SSR, AFLR and 10 microsatellite loci polymorphisms show some significant differences in the allocation of the analysed accessions. The presence of Tvv1 in all the analysed genotypes suggests that this retrotransposon element is widespread in Vitis genus and indicates that it was active before Vitis speciation and contributed the evolution of this genus.

Retrotransposon Based Molecular Markers in Grapevine Cultivars and Clones Identification

D'ONOFRIO, CLAUDIO;GIORDANI, TOMMASO;NATALI, LUCIA;SCALABRELLI, GIANCARLO;CAVALLINI, ANDREA
2009

Abstract

In this study we analysed the polymorphisms of retrotransposon based molecular markers (IRAR REMAR SSAP) in 22 Vitis genotypes: 16 Vitis vinifera genotypes (eight different 'Sangiovese' clones, four different 'Niellucciu' clones, 'Prugnolo gentile', 'Ciliegiolo', 'Trebbiano toscano', 'Vermentino') Vitis vinifera subsp. sylvestris, Vitis rupestis du Lot, Vitis cinerea, Vitis cordifolia, Vitis champinii, and Vitis rotundifolia. In particular, we studied the polymorphisms of three different retrotransposons found in the grapevine genome, two copia-like, Vine-I and, Tvv1, and one gypsy-like, Gret1. Among these, Tvv1 showed the highest polymorphisms and allowed us to clearly distinguish among Vitis species and Vitis vinifera varieties analysed. On the same genotypes, we also analysed some SSR, I-SSR, and AFLP polymorphisms. The phylogenetic tree obtained from the Tvv1 polymorphisms and those obtained from I-SSR, AFLR and 10 microsatellite loci polymorphisms show some significant differences in the allocation of the analysed accessions. The presence of Tvv1 in all the analysed genotypes suggests that this retrotransposon element is widespread in Vitis genus and indicates that it was active before Vitis speciation and contributed the evolution of this genus.
D'Onofrio, Claudio; DE LORENZIS, G; Giordani, Tommaso; Natali, Lucia; Scalabrelli, Giancarlo; Cavallini, Andrea
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11568/135293
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