A method is described for detecting the gamma-glutamyltransferase enzyme isoforms in a sample of biological fluid, such as for example plasma or serum. The method comprises an HPLC separation step of the sample proteins based on the molecular size and a second step for detecting the GGT isoforms by post-column reaction with a GGT enzyme substrate capable of generating a detectable final product, preferably by spectrophotometric or spectrofluorimetric means. The GGT isoforms can be separated by ultracentrifugation, thereby obtaining three enzymatic isoforms characterized by molecular weights of approximately 2000, 940, and 140 KDa, respectively.
Method of detecting and separating serum gamma-glutamyltransferase (GGT) isoforms in a sample of biological fluid and the enzyme isoforms thereby obtained.
PAOLICCHI, ALDO;POMPELLA, ALFONSO;FRANZINI, MARIA;BARSACCHI, RENATA;EMDIN, MICHELE;
2010-01-01
Abstract
A method is described for detecting the gamma-glutamyltransferase enzyme isoforms in a sample of biological fluid, such as for example plasma or serum. The method comprises an HPLC separation step of the sample proteins based on the molecular size and a second step for detecting the GGT isoforms by post-column reaction with a GGT enzyme substrate capable of generating a detectable final product, preferably by spectrophotometric or spectrofluorimetric means. The GGT isoforms can be separated by ultracentrifugation, thereby obtaining three enzymatic isoforms characterized by molecular weights of approximately 2000, 940, and 140 KDa, respectively.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
