Thiopurine prodrugs are antiviral chemicals used in medical therapy whose mechanisms of action are asso- ciated with inhibition of purine biosynthesis. In terms of plant chemotherapy, previous research of 6-mercap- topurine (MP) administration in tobacco tissue culture infected by Tobacco mosaic virus (TMV) showed no inhibition of virus activity. Currently, not enough data exist to confirm thiopurine drug ineffectiveness against viruses in the plant kingdom. This paper presents a screening of MP, 6-methylmercaptopurine riboside (MMPR), 6-thioguanine (6-TG) and 1-amino-6-mercaptopurine (1A-MP) against TMV and Cucumber mosaic virus (CMV) in in vitro tobacco explants and against Grapevine leafroll-associated virus 3 (GLRaV 3)in in vitro grapevine explants. ELISA and RT-PCR were used to evaluate antiviral activity. Higher toxicity levels of MP derivatives, compared to MP, were noted in tobacco and grapevine explants. 1A-MP or 6-TG treatment resulted CMV and GLRaV 3 virus-eradi- cated explants as obtained with Inosine 5¢-monophos- phate dehydrogenase inhibitors, whereas TMV was not eradicated by any of the studied drugs.
Thiopurine prodrugs for plant chemotherapy purposes
PANATTONI, ALESSANDRA;TRIOLO, ENRICO
2010-01-01
Abstract
Thiopurine prodrugs are antiviral chemicals used in medical therapy whose mechanisms of action are asso- ciated with inhibition of purine biosynthesis. In terms of plant chemotherapy, previous research of 6-mercap- topurine (MP) administration in tobacco tissue culture infected by Tobacco mosaic virus (TMV) showed no inhibition of virus activity. Currently, not enough data exist to confirm thiopurine drug ineffectiveness against viruses in the plant kingdom. This paper presents a screening of MP, 6-methylmercaptopurine riboside (MMPR), 6-thioguanine (6-TG) and 1-amino-6-mercaptopurine (1A-MP) against TMV and Cucumber mosaic virus (CMV) in in vitro tobacco explants and against Grapevine leafroll-associated virus 3 (GLRaV 3)in in vitro grapevine explants. ELISA and RT-PCR were used to evaluate antiviral activity. Higher toxicity levels of MP derivatives, compared to MP, were noted in tobacco and grapevine explants. 1A-MP or 6-TG treatment resulted CMV and GLRaV 3 virus-eradi- cated explants as obtained with Inosine 5¢-monophos- phate dehydrogenase inhibitors, whereas TMV was not eradicated by any of the studied drugs.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
