Introduction: Molecular-based subtypes consisting of two receptorpositive (OR and/or PR) types (luminal-like A and luminal-like B) and three hormone receptor-negative types (HER-2-expressing, basal-like and normal-like) have been investigated in mammary gland tumours of the dog by immunohistochemistry (IHC). The aim of the present study was to apply an IHC panel to a series of 12 rabbit mammary tumours to verify molecular-based classification in this species and to evaluate the rabbit as an animal model of human breast cancer. Materials and Methods: Three hyperplastic mammary glands, two adenomas and 10 adenocarcinomas underwent IHC with the following panel of antibodies: oestrogen receptor-a (OR-a), HER-2 and cytokeratin 5/6 (CK 5/6). Luminal-like (A and B), basal-like, HER-2 and normal-like patterns were determined. Results: Six cases were OR-a positive (two hyperplastic, two adenomas and two adenocarcinomas). HER-2 positivity was observed in two cases (one adenoma, one adenocarcinoma). Cytokeratin 5/6 positivity was identified in three cases (one adenoma, two adenocarcinomas). Among the 10 carcinomas, five normal-like, three luminallike A, one basal-like and one HER-2 patterns were identified. Conclusions: In the rabbit all five molecular subtypes were identified with normal-like and luminal-like as most represented. The rabbit appears to be the best animal model for human breast cancer if basal and luminal-like patterns are considered.
Phenotypic characterization of spontaneous mammary tumours in per rabbits
RESSEL, LORENZO;BENAZZI, CINZIA;POLI, ALESSANDRO;SARLI PROF., GIUSEPPE
2010-01-01
Abstract
Introduction: Molecular-based subtypes consisting of two receptorpositive (OR and/or PR) types (luminal-like A and luminal-like B) and three hormone receptor-negative types (HER-2-expressing, basal-like and normal-like) have been investigated in mammary gland tumours of the dog by immunohistochemistry (IHC). The aim of the present study was to apply an IHC panel to a series of 12 rabbit mammary tumours to verify molecular-based classification in this species and to evaluate the rabbit as an animal model of human breast cancer. Materials and Methods: Three hyperplastic mammary glands, two adenomas and 10 adenocarcinomas underwent IHC with the following panel of antibodies: oestrogen receptor-a (OR-a), HER-2 and cytokeratin 5/6 (CK 5/6). Luminal-like (A and B), basal-like, HER-2 and normal-like patterns were determined. Results: Six cases were OR-a positive (two hyperplastic, two adenomas and two adenocarcinomas). HER-2 positivity was observed in two cases (one adenoma, one adenocarcinoma). Cytokeratin 5/6 positivity was identified in three cases (one adenoma, two adenocarcinomas). Among the 10 carcinomas, five normal-like, three luminallike A, one basal-like and one HER-2 patterns were identified. Conclusions: In the rabbit all five molecular subtypes were identified with normal-like and luminal-like as most represented. The rabbit appears to be the best animal model for human breast cancer if basal and luminal-like patterns are considered.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
