The oxidation mechanism of hematoxylin was studied in phosphate buffers and 0.1 M KCl by cyclic voltammetry and UV-Vis spectroscopy under deaerated conditions. The redox potential of hematoxylin in buffered solution strongly depends on pH. A two electron oxidation is preceded by deprotonation. The homogeneous rate of deprotonation process of hematoxylin in 0.1 M phosphate buffer is k(d) = (2.5 +/- 0.1) x 10(4) s(-1). The cyclic voltammetry under unbuffered conditions shows the distribution of various dissociation forms of hematoxylin. The dissociation constants pK(1) = 4.7 +/- 0.2 and pK(2) = 9.6 +/- 0.1 were determined using UV-Vis spectroscopy. The final oxidation product was identified by gas chromatography with mass spectrometry detection as hemathein. The distribution of oxidation products differs under buffered and unbuffered conditions. The dye degradation in natural unbuffered environment yields hemathein and hydroxyhematoxylin, which is absent in buffered solution.
|Autori:||R. SOKOLOVÁ; DEGANO I; M. HROMADOVÁ; J. BULÍCKOVÁ; M. GÁL AND M. VALÁEK|
|Titolo:||Oxidation pathways of natural dye hematoxylin in aqueous solution|
|Anno del prodotto:||2010|
|Digital Object Identifier (DOI):||10.1135/cccc2010096|
|Appare nelle tipologie:||1.1 Articolo in rivista|