Virgin olive oil (VOO) has nutritional and sensory characteristics that make it unique and a basic component of the Mediterranean diet. Its importance is mainly attributed to its richness in polyphenols, which act as natural antioxidants and may contribute to the prevention of several human diseases. In this paper we report the detn. and quantification of oleocanthal, one of the main substances responsible for the bitter taste of olive oil, together with a quali-quant. anal. by HPLC anal. methods of phenolics from Cilento VOO and olive oil pomace. The total phenolic content was also detd. and the in vitro antioxidant and free-radical scavenging activities by DPPH test was evaluated. A superoxide anion enzymic assay was also carried out and the results were confirmed by the inhibition of xanthine oxidase activity assay. The possible protective role played by VOO secoiridoids on injurious effects of reactive oxygen metabolites on the intestinal epithelium, using Caco-2 human cell line, was investigated.
Phenolic compounds in olive oil and olive pomace from Cilento (Campania, Italy) and their antioxidant activity
BRACA, ALESSANDRA;
2010-01-01
Abstract
Virgin olive oil (VOO) has nutritional and sensory characteristics that make it unique and a basic component of the Mediterranean diet. Its importance is mainly attributed to its richness in polyphenols, which act as natural antioxidants and may contribute to the prevention of several human diseases. In this paper we report the detn. and quantification of oleocanthal, one of the main substances responsible for the bitter taste of olive oil, together with a quali-quant. anal. by HPLC anal. methods of phenolics from Cilento VOO and olive oil pomace. The total phenolic content was also detd. and the in vitro antioxidant and free-radical scavenging activities by DPPH test was evaluated. A superoxide anion enzymic assay was also carried out and the results were confirmed by the inhibition of xanthine oxidase activity assay. The possible protective role played by VOO secoiridoids on injurious effects of reactive oxygen metabolites on the intestinal epithelium, using Caco-2 human cell line, was investigated.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
