In the frame of a phytosanitary survey on a Lilium varietal collection, held at CRA-VIV in Pescia (PT), leaf samples were collected from 60 plants belonging to 20 varieties and tested by RT-PCR for the subsequent viruses: Cucumber Mosaic Virus (CMV), Lilium Simptomless Virus (LSV) and Lilium Mottle Virus (LMoV). Three plants of each variety were sampled in late spring-early summer 2010. Total RNA was extracted with a modified protocol using the “RNeasy Plant mini kit” (Qiagen) and retro-transcribed into cDNA. Diagnosis of CMV, LSV and LMoV was at first performed with specific protocols described in literature; such protocols were then uniformed to unique amplification conditions in order to allow a faster singleplex detection of the three viruses. Results obtained with the new amplification conditions perfectly matched the results of the standard protocols, confirming the specificity and sensitivity of the tests. On 60 samples, 40 (66.7%), belonging to 17 varieties, were infected with CMV, 56 (93.3%), belonging to 20 varieties, resulted positive to LSV and 4 (6.7%), belonging to 2 varieties, were infected with LMoV. The same 60 samples were successively tested with RT-PCR assays for two tospoviruses: Impatiens Necrotic Spot Virus (INSV) and Tomato Spotted Wilt Virus (TSWV). All of the samples resulted negative to both tospoviruses
Survey of viruses infecting a Lilium varietal collection in Tuscany
DELLA BARTOLA, MICHELE;MATERAZZI, ALBERTO;
2011-01-01
Abstract
In the frame of a phytosanitary survey on a Lilium varietal collection, held at CRA-VIV in Pescia (PT), leaf samples were collected from 60 plants belonging to 20 varieties and tested by RT-PCR for the subsequent viruses: Cucumber Mosaic Virus (CMV), Lilium Simptomless Virus (LSV) and Lilium Mottle Virus (LMoV). Three plants of each variety were sampled in late spring-early summer 2010. Total RNA was extracted with a modified protocol using the “RNeasy Plant mini kit” (Qiagen) and retro-transcribed into cDNA. Diagnosis of CMV, LSV and LMoV was at first performed with specific protocols described in literature; such protocols were then uniformed to unique amplification conditions in order to allow a faster singleplex detection of the three viruses. Results obtained with the new amplification conditions perfectly matched the results of the standard protocols, confirming the specificity and sensitivity of the tests. On 60 samples, 40 (66.7%), belonging to 17 varieties, were infected with CMV, 56 (93.3%), belonging to 20 varieties, resulted positive to LSV and 4 (6.7%), belonging to 2 varieties, were infected with LMoV. The same 60 samples were successively tested with RT-PCR assays for two tospoviruses: Impatiens Necrotic Spot Virus (INSV) and Tomato Spotted Wilt Virus (TSWV). All of the samples resulted negative to both tospovirusesI documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
