In many functional genomic studies, cell suspension cultures have been very useful experimental tools. Experiments with grape cell suspensions are quick and convenient compared to studies on whole vines and berries, they make it easy to control the environment conditions and to manipulate with accuracy the experiment conditions, and it is possible to carry out experiments all year. The aim of this project is the discovery and functional characterizations of genes involved in flavour biosynthetic pathways by elicitation of grape cell suspension. Here we report the optimization of sesquiterpenes production in the methyl-jasmonate (MeJA) elicited grape cell suspensions that have been utilized for real-time RT-PCR and microarray analysis experiments reported in D’Onofrio et al., 2009.
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