BACKGROUND: Thyroid gland is highly dependent on dietary intake of iodine for normal function, so it is particularly subjected to "endocrine disruptor" action. The human sodium/iodide symporter (hNIS) is an integral plasma membrane glycoprotein mediating the active transport of iodide into thyroid follicular cells, a crucial step for thyroid hormone biosynthesis. Beyond to perchlorate and thyocianate ions a few other inhibitors of iodide uptake have been described. AIM: The aim of this study was to investigate if 10 substances usually used as drugs in clinical practice were able to inhibit NIS-mediated iodide uptake in vitro. MATERIALS AND METHODS: A CHO cell line stably expressing hNIS was used to test any inhibition of NIS-mediated iodide uptake exerted by drugs. Perchlorate and thyocianate ions were used as positive controls. RESULTS: None of the analyzed substances was able to significantly inhibit iodide uptake in our system. As we expected, perchlorate and thyocianate ions were able to inhibit iodide uptake in a dose-dependent manner. CONCLUSIONS: In conclusion, we carried out an in vitro assay to evaluate the potential inhibitory effect of common drugs on NISmediated iodide uptake by using CHO-hNIS cells. None of the analyzed substances was able to inhibit iodide uptake; only perchlorate and thyocianate were able to inhibit iodide uptake in a dose-dependent manner.
|Autori:||AGRETTI P; DIMIDA A; DE MARCO G; FERRARINI E; RODRÌGUEZ GONZÀLEZ JC; SANTINI F; VITTI P; PINCHERA A; TONACCHERA M|
|Titolo:||STUDY OF POTENTIAL INHIBITORS OF THYROID IODIDE UPTAKE BY USING CHO CELLS STABLY EXPRESSING THE HUMAN SODIUM/IODIDE SYMPORTER (hNIS) PROTEIN|
|Anno del prodotto:||2011|
|Digital Object Identifier (DOI):||10.3275/7046|
|Appare nelle tipologie:||1.1 Articolo in rivista|