Grapevine yellows (GY) are an important disease of Vitis vinifera caused by phytoplasmas. In Italy, GY are mostly represented by Flavescence dorée (FD) and Bois noir (BN), respectively associated with infection by 16SrV and 16SrXII phytoplasma ribosomal groups. In the frame of a regional strategy aimed to monitor GY presence, an extensive survey was carried out in late summer 2010, collecting 629 leaf samples from the inspected commercial vineyards. Samples were preferably collected from plants showing symptoms referable to GY diseases. Each sampled plant was marked and localised by “Global Positioning System - GPS”, in order to allow tracking of FD positive samples for uprooting. DNA extracts were tested with a Real-time PCR assay for the diagnosis of BN and FD phytoplasmas. The results confirmed that BN is largely the most important GY in Tuscany, with an ubiquitous distribution. In fact, it was present in almost every surveyed vineyard with an overall incidence of 53,6% (337 out of 629 plants tested). The presence of FD was recorded in 39 samples distributed in 4 provinces including: Massa-Carrara, Florence, Pistoia and Lucca. An alarming situation was observed in this latter province, where 23 out of 74 plants (31,1%) were infected by FD. On the basis of these results, further investigations on insect vectors, natural reservoirs and molecular characterization of grapevine infecting phytoplasmas are needed in order to monitor and understand the epidemiology of GY diseases in Tuscany.
Further data on the distribution of grapevine yellows in Tuscany
DELLA BARTOLA, MICHELE;MATERAZZI, ALBERTO
2011-01-01
Abstract
Grapevine yellows (GY) are an important disease of Vitis vinifera caused by phytoplasmas. In Italy, GY are mostly represented by Flavescence dorée (FD) and Bois noir (BN), respectively associated with infection by 16SrV and 16SrXII phytoplasma ribosomal groups. In the frame of a regional strategy aimed to monitor GY presence, an extensive survey was carried out in late summer 2010, collecting 629 leaf samples from the inspected commercial vineyards. Samples were preferably collected from plants showing symptoms referable to GY diseases. Each sampled plant was marked and localised by “Global Positioning System - GPS”, in order to allow tracking of FD positive samples for uprooting. DNA extracts were tested with a Real-time PCR assay for the diagnosis of BN and FD phytoplasmas. The results confirmed that BN is largely the most important GY in Tuscany, with an ubiquitous distribution. In fact, it was present in almost every surveyed vineyard with an overall incidence of 53,6% (337 out of 629 plants tested). The presence of FD was recorded in 39 samples distributed in 4 provinces including: Massa-Carrara, Florence, Pistoia and Lucca. An alarming situation was observed in this latter province, where 23 out of 74 plants (31,1%) were infected by FD. On the basis of these results, further investigations on insect vectors, natural reservoirs and molecular characterization of grapevine infecting phytoplasmas are needed in order to monitor and understand the epidemiology of GY diseases in Tuscany.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
