In the frame of a phytovirological survey on 21 species/varieties of Hydrangea spp. and on 2 species of the similar genus Schizophragma held at CRA-VIV in Pescia (PT), 44 plants were tested by RT-PCR in order to detect possible infections by Hydrangea Ringspot Virus (HdRSV). Leaf samples were collected in late spring – early summer 2010 from symptomatic (crinkling, rolling and asymmetry of leaves, decrease in number of florets per inflorescence, severe dwarfing and ‘running out’) plants. Total RNA was extracted with a modified protocol using the “RNeasy Plant mini kit” (Qiagen) and retro-transcribed into cDNA. RT-PCR analysis were performed with protocols already described in literature. Results obtained showed that 15 out of 44 plants (34.1%), belonging to 11 cultivars of 5 different species of Hydrangea were infected with HdRSV. The amplicon obtained was then sequenced and proved to share a high similarity with the HdRSV sequences present in GenBank. All of the plants belonging to the Schizophragma genus provided negative results
Occurence of Hydrangea ring spot virus in a Hydrangea varietal collection
DELLA BARTOLA, MICHELE;MATERAZZI, ALBERTO;
2011-01-01
Abstract
In the frame of a phytovirological survey on 21 species/varieties of Hydrangea spp. and on 2 species of the similar genus Schizophragma held at CRA-VIV in Pescia (PT), 44 plants were tested by RT-PCR in order to detect possible infections by Hydrangea Ringspot Virus (HdRSV). Leaf samples were collected in late spring – early summer 2010 from symptomatic (crinkling, rolling and asymmetry of leaves, decrease in number of florets per inflorescence, severe dwarfing and ‘running out’) plants. Total RNA was extracted with a modified protocol using the “RNeasy Plant mini kit” (Qiagen) and retro-transcribed into cDNA. RT-PCR analysis were performed with protocols already described in literature. Results obtained showed that 15 out of 44 plants (34.1%), belonging to 11 cultivars of 5 different species of Hydrangea were infected with HdRSV. The amplicon obtained was then sequenced and proved to share a high similarity with the HdRSV sequences present in GenBank. All of the plants belonging to the Schizophragma genus provided negative resultsI documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
