Grapevine germplasm, including 38 of the main Portuguese cultivars and three foreign cultivars, Pinot Noir, Pinot Blanc and Chasselas, used as a reference, and 37 trueto- type clones from the Alvarinho, Arinto, Loureiro, Moscatel Galego Branco, Trajadura and Vinha˜o cultivars were studied using AFLP and three retrotransposon-based molecular techniques, IRAP, REMAP and SSAP. To study the retrotransposon-based polymorphisms, 18 primers based on the LTR sequences of Tvv1, Gret1 and Vine-1 were used. In the analysis of 41 cultivars, 517 IRAP, REMAP, AFLP and SSAP fragments were obtained, 83% of which were polymorphic. For IRAP, only the Tvv1Fa primer amplified DNA fragments. In the REMAP analysis, the Tvv1Fa-Ms14 primer combination only produced polymorphic bands, and the Vine-1 primers produced mainly ISSR fragments. The highest number of polymorphic fragments was found for AFLP. Both AFLP and SSAP showed a greater capacity for identifying clones, resulting in 15 and 9 clones identified, respectively. Together, all of the techniques allowed for the identification of 54% of the studied clones, which is an important step in solving one of the challenges that viticulture currently faces.
|Autori:||Castro I; D'Onofrio C; Martín JP; Ortiz JM; De Lorenzis G; Ferreira V; Pinto-Carnide O.|
|Titolo:||Effectiveness of AFLPs and retrotransposon-based markers for grapevine cultivars and clones identification|
|Anno del prodotto:||2012|
|Digital Object Identifier (DOI):||10.1007/s12033-011-9470-y|
|Appare nelle tipologie:||1.1 Articolo in rivista|