Introduction: Studies in human and cows report the oxidative status in the feto-placental system at birth1,2,5. No reports have been published on this item in equine specie. The aim of this study was to assess the hydroxyl radicals (d-ROMs) and plasmatic antioxidants (BAP) concentrations in mare, umbilical cord and foal to evaluate oxidative stress immediately after birth. Materials and methods: plasma samples were obtained from 10 healthy mares, their foals and umbilical cords. All deliveries were euthocic and all foals showed an APGAR score ≥ 7. Every sample was assessed by spectrophotometric tests (Diacron International, Italy). Data were analyzed by ANOVA test, LSD Multiple Range Test and Multiple Correlation Test. Differences were significant if P<0.05. Results: no statistical differences were obtained in plasma d-ROMs between mares and umbilical cords (247,7±42,9 U.CARR and 201,7±67,8 U.CARR, respectively), while d-ROMs in plasma foals were significantly lower (178,6±27,3 U.CARR.) (P<0,01). Plasma BAP values were significantly different between mares, umbilical cords and foals (2464±199 μmol/l, 2215±191, μmol/l and 2324±214 μmol/l, respectively) (P<0,05). Besides, a significant correlation (r=0.65, P<0,05) was observed in plasma BAP between mares and umbilical cords. Discussion and conclusions: several papers confirmed the condition of oxidative stress at delivery, but data are often discordant and fragmented. Few data are reported in human literature, about differences on oxidative-antioxidative balance between women and their newborn babies at birth. In our study, results concerning d-ROMs concentration are in line with other authors who referred lower umbilical cord than mother values of Reactice Oxygen Species (ROS) and malondialdeide (MDA), respectively2,5. A recent study performed in cattle, reported a higher intensity of lipid peroxidation in veals’ than in cows’ plasma immediately after calving. This value decreased considerably in the first 24 hours of life1. Thus, antioxidant concentration results higher in umbilical cord plasma than in mother plasma, both in human and cows1,2. We also confirm this trend in horses. Recent studies show a significant difference in oxidant-antioxidant markers being higher in caesarean sections compared with vaginal delivery, as well as pre-term infants compared to term ones3. Moreover, the development of many neonatal diseases appears associated with high levels of oxidative stress markers in umbilical cord4. For this reason, standardization and a large clinical use of these biomarkers might contribute in the early detection, management and therapy of several neonatal diseases in all species.
Mares and foals concentrations of hydroxil radicals and plasmatic antioxidants: preliminary results
PASQUINI, ANNA;BIAGI, GIULIA;CORAZZA, MICHELE;SGORBINI, MICAELA
2012-01-01
Abstract
Introduction: Studies in human and cows report the oxidative status in the feto-placental system at birth1,2,5. No reports have been published on this item in equine specie. The aim of this study was to assess the hydroxyl radicals (d-ROMs) and plasmatic antioxidants (BAP) concentrations in mare, umbilical cord and foal to evaluate oxidative stress immediately after birth. Materials and methods: plasma samples were obtained from 10 healthy mares, their foals and umbilical cords. All deliveries were euthocic and all foals showed an APGAR score ≥ 7. Every sample was assessed by spectrophotometric tests (Diacron International, Italy). Data were analyzed by ANOVA test, LSD Multiple Range Test and Multiple Correlation Test. Differences were significant if P<0.05. Results: no statistical differences were obtained in plasma d-ROMs between mares and umbilical cords (247,7±42,9 U.CARR and 201,7±67,8 U.CARR, respectively), while d-ROMs in plasma foals were significantly lower (178,6±27,3 U.CARR.) (P<0,01). Plasma BAP values were significantly different between mares, umbilical cords and foals (2464±199 μmol/l, 2215±191, μmol/l and 2324±214 μmol/l, respectively) (P<0,05). Besides, a significant correlation (r=0.65, P<0,05) was observed in plasma BAP between mares and umbilical cords. Discussion and conclusions: several papers confirmed the condition of oxidative stress at delivery, but data are often discordant and fragmented. Few data are reported in human literature, about differences on oxidative-antioxidative balance between women and their newborn babies at birth. In our study, results concerning d-ROMs concentration are in line with other authors who referred lower umbilical cord than mother values of Reactice Oxygen Species (ROS) and malondialdeide (MDA), respectively2,5. A recent study performed in cattle, reported a higher intensity of lipid peroxidation in veals’ than in cows’ plasma immediately after calving. This value decreased considerably in the first 24 hours of life1. Thus, antioxidant concentration results higher in umbilical cord plasma than in mother plasma, both in human and cows1,2. We also confirm this trend in horses. Recent studies show a significant difference in oxidant-antioxidant markers being higher in caesarean sections compared with vaginal delivery, as well as pre-term infants compared to term ones3. Moreover, the development of many neonatal diseases appears associated with high levels of oxidative stress markers in umbilical cord4. For this reason, standardization and a large clinical use of these biomarkers might contribute in the early detection, management and therapy of several neonatal diseases in all species.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
