The present study was performed to determine if there is an association between thegenotype and transmission of HHV-8 types A and C. These HHV subtypes are prevalent in the area of northern Sardinia, which is an island off west Italy’s mainland that has a high HHV-8 seroprevalence (35%). Blood and saliva samples from 30 patients with classic Kaposi’s sarcomawho were lifetime residentsofNorth Sardiniawere analysed to identify the HHV-8 genotype and quantitate the viral load. Genotype A, especially the A1 subtype, was the most prevalent (9/30 patients)and had a significantly higher viral load in the saliva compared to blood (P=0.029);in contrast, HHV type C had a higher viral load in the blood. To determine if there is a correlation between the viral genotype and cellular tropism, type A1 and C3 HHV-8 viral particleswere obtained from latent chronically infected patients.The patient viral samples were cultured in BCBL1 and BC3 cell linesand used to infect HEK293epithelial origin cells and PBMCsin vitro. The data indicate that theA1 HHV-8 genotype is tropic and replicates at higher levels in the epithelial cell lines.
In vivo and in vitro evidence for an association between hhv-8 route-specific transmission and the virus genotype.
CECCHERINI-NELLI, LUCA
2012-01-01
Abstract
The present study was performed to determine if there is an association between thegenotype and transmission of HHV-8 types A and C. These HHV subtypes are prevalent in the area of northern Sardinia, which is an island off west Italy’s mainland that has a high HHV-8 seroprevalence (35%). Blood and saliva samples from 30 patients with classic Kaposi’s sarcomawho were lifetime residentsofNorth Sardiniawere analysed to identify the HHV-8 genotype and quantitate the viral load. Genotype A, especially the A1 subtype, was the most prevalent (9/30 patients)and had a significantly higher viral load in the saliva compared to blood (P=0.029);in contrast, HHV type C had a higher viral load in the blood. To determine if there is a correlation between the viral genotype and cellular tropism, type A1 and C3 HHV-8 viral particleswere obtained from latent chronically infected patients.The patient viral samples were cultured in BCBL1 and BC3 cell linesand used to infect HEK293epithelial origin cells and PBMCsin vitro. The data indicate that theA1 HHV-8 genotype is tropic and replicates at higher levels in the epithelial cell lines.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
