An Additive Manufacturing technique for the fabrication of three-dimensional polymeric scaffolds, based on wet-spinning of poly(epsilon-caprolactone) (PCL) or PCL/hydroxyapatite (HA) solutions, was developed. The processing conditions to fabricate scaffolds with a layer-by-layer approach were optimized by studying their influence on fibres morphology and alignment. Two different scaffold architectures were designed and fabricated by tuning inter-fibre distance and fibres staggering. The developed scaffolds showed good reproducibility of the internal architecture characterized by highly porous, aligned fibres with an average diameter in the range 200-250 mu m. Mechanical characterization showed that the architecture and HA loading influenced the scaffold compressive modulus and strength. Cell culture experiments employing MC3T3-E1 preosteoblast cell line showed good cell adhesion, proliferation, alkaline phosphatase activity and bone mineralization on the developed scaffolds.
|Autori:||Puppi D; Mota C; Gazzarri M; Dinucci D; Gloria A; Myrzabekova M; Ambrosio L; Chiellini F|
|Titolo:||Additive manufacturing of wet-spun polymeric scaffolds for bone tissue engineering|
|Anno del prodotto:||2012|
|Digital Object Identifier (DOI):||10.1007/s10544-012-9677-0|
|Appare nelle tipologie:||1.1 Articolo in rivista|