An electroanalytical method was developed for the determination of the phytohormone indole-3-acetic acid in water using a glassy-carbon electrode. The differential pulse voltammetry was combined with a purification step which involved the extraction of plant tissue and the separation of plant pigments by passing through column Oasis MCX. Indole-3-acetic acid can be determined under optimum conditions with a limit of quantification (LOQ) 2.7 106 mol L1. The linearity range was from 1.8106 to 6.6104 mol L1. The electroanalytical determination of indole-3-acetic acid is selective, fast and economic. The presence of other phytohormones as abscisic acid and cytokinins as isopentenyladenine, isopentenyladenosine, trans-zeatin, zeatin riboside does not interfere the determination of indole-3-acetic acid in plant sample.
|Autori:||Jana Bulickova; Romana Sokolova; GIANNARELLI S; Beatrice Muscatello|
|Titolo:||Determination of Plant Hormone Indole-3-Acetic Acid in Aqueous Solution|
|Anno del prodotto:||2013|
|Digital Object Identifier (DOI):||10.1002/elan.201200394|
|Appare nelle tipologie:||1.1 Articolo in rivista|