Plants have developed a constitutive defence system against pest attacks, which involves the expression of a set of inhibitors acting on heterologous amylases of different origins. Investigating the soluble protein complement of the hulled wheat emmer we have isolated and characterized a heterotetrameric α-amylase inhibitor (ETI). Based on mass spectroscopy data, it is an assembly of proteins highly similar to the CM2/CM3/CM16 found in durum wheat. Our data indicate that these proteins can also inhibit exogenous α-amylases in binary assemblies. The calculated dissociation constants (Ki) for the pancreatic porcine amylase- and human salivary amylase-ETI complexes are similar to those found in durum and soft wheat. Homology modeling of the CM subunits indicate structural similarities with other proteins belonging to the cereal family of trypsin/α-amylase inhibitors; a possible homology modeled structure for a tetrameric assembly of the subunits is proposed.

A heterotetrameric alpha-amylase inhibitor from emmer (Triticum dicoccon Schrank) seeds

CAPOCCHI, ANTONELLA;FONTANINI, DEBORA
2013-01-01

Abstract

Plants have developed a constitutive defence system against pest attacks, which involves the expression of a set of inhibitors acting on heterologous amylases of different origins. Investigating the soluble protein complement of the hulled wheat emmer we have isolated and characterized a heterotetrameric α-amylase inhibitor (ETI). Based on mass spectroscopy data, it is an assembly of proteins highly similar to the CM2/CM3/CM16 found in durum wheat. Our data indicate that these proteins can also inhibit exogenous α-amylases in binary assemblies. The calculated dissociation constants (Ki) for the pancreatic porcine amylase- and human salivary amylase-ETI complexes are similar to those found in durum and soft wheat. Homology modeling of the CM subunits indicate structural similarities with other proteins belonging to the cereal family of trypsin/α-amylase inhibitors; a possible homology modeled structure for a tetrameric assembly of the subunits is proposed.
2013
Capocchi, Antonella; Muccilli, V.; Cunsolo, V.; Saletti, R.; Foti, S.; Fontanini, Debora
File in questo prodotto:
File Dimensione Formato  
A heterotetrameric alpha-amylase inhibitor from emmer (Triticum dicoccon Schrank) seeds.pdf

solo utenti autorizzati

Descrizione: Articolo principale
Tipologia: Documento in Pre-print
Licenza: NON PUBBLICO - Accesso privato/ristretto
Dimensione 2.7 MB
Formato Adobe PDF
2.7 MB Adobe PDF   Visualizza/Apri   Richiedi una copia
A heterotetrameric alpha-amylase inhibitor from emmer (Triticum dicoccon Schrank) seeds - SI.pdf

solo utenti autorizzati

Descrizione: Tabella Supplementare
Tipologia: Altro materiale allegato
Licenza: NON PUBBLICO - Accesso privato/ristretto
Dimensione 47.88 kB
Formato Adobe PDF
47.88 kB Adobe PDF   Visualizza/Apri   Richiedi una copia
Heterotetrameric - Post print.pdf

accesso aperto

Tipologia: Documento in Post-print
Licenza: Creative commons
Dimensione 8.01 MB
Formato Adobe PDF
8.01 MB Adobe PDF Visualizza/Apri

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11568/159550
Citazioni
  • ???jsp.display-item.citation.pmc??? 3
  • Scopus 24
  • ???jsp.display-item.citation.isi??? 22
social impact