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EnglishA rapid and sensitive HPLC method has been developed for the quantification of mirtazapine (MRZ), a noradrenergic and specific serotonergic inhibitor antidepressant (NaSSA) and its two major metabolites N-desmethylmirtazapine (NDM) and 8-hydroxymirtazapine (8-OHM) in human plasma. The separation was achieved using Chromolith C18 columns and a mobile phase of acetonitrile: phosphate buffer (pH=3, 20:80, v/v) in isocratic mode at a flow rate of 2 ml/min. A fluorescence detector was set at 290 and 350 nm for excitation and emission, respectively. Zolpidem was used as the internal standard. Liquid-liquid extraction was applied for sample clean up. All analytes were eluted in less than 5 minutes with LOQ of 1 ng/ml for MRZ and 2 ng/ml for both NDM and 8-OHM . The developed method was successfully applied to quantify MRZ and its metabolites in plasma of a healthy volunteer.
| Autori interni: | |
| Autori: | H., Lavasani; Giorgi, Mario; B., Sheikholeslami; M. R., Rouini |
| Titolo: | A RAPID AND SENSITIVE HPLC-FLUORESCENCE METHOD FOR DETERMINATION OF MIRTAZAPINE AND ITS TWO MAJOR METABOLITES IN HUMAN PLASMA |
| Anno del prodotto: | 2014 |
| Appare nelle tipologie: | 1.1 Articolo in rivista |
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http://hdl.handle.net/11568/159683
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