Bellevalia romana (L.) Rchb., a monocotyledonous plant characterized by few (2n=2x=8) and very large chromosomes, may regenerate plants both via adventitious bud differentiation and via somatic embryogenesis in suspension cultures. Cytological analysis of numerous embryoids at different developmental stages in most of them revealed a mixoploid and aneusomatic condition which increased during embryo growth. Only globular proembryos were all entirely diploid. The results are discussed in relation to: i) the origin of the embryos; ii)de novo induction and increase in abnormal metaphases during embryogenesis; iii) embryo inability to develop into plant owing to chromosomal mosaicism.
INVITRO CULTURE OF BELLEVALIA-ROMANA (L) RCHB .3. CYTOLOGICAL STUDY OF SOMATIC EMBRYOS
CAVALLINI, ANDREA;CREMONINI, ROBERTO;
1987-01-01
Abstract
Bellevalia romana (L.) Rchb., a monocotyledonous plant characterized by few (2n=2x=8) and very large chromosomes, may regenerate plants both via adventitious bud differentiation and via somatic embryogenesis in suspension cultures. Cytological analysis of numerous embryoids at different developmental stages in most of them revealed a mixoploid and aneusomatic condition which increased during embryo growth. Only globular proembryos were all entirely diploid. The results are discussed in relation to: i) the origin of the embryos; ii)de novo induction and increase in abnormal metaphases during embryogenesis; iii) embryo inability to develop into plant owing to chromosomal mosaicism.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.