Simultaneous HHV-6A infection can activate HIV-1 latency and promote AIDS progression, but in this process the effects of HHV-6A induced soluble mediators on HIV-1 have not been studied yet. Recently, supernatants of HSB-2 cultures infected with HHV-6A and/or treated with endotoxin have been filtered virus free at time intervals until the cytopathic effect developed. Biological activity of some cytokines which might participate in HIV-1 activation was quantitated. Filtered supernatants were mixed into CEM-ss cultures, which had been HIV-1 infected at 1:1 cell:virus ratio, subsequently HIV-1 replication was quantitated and compared to controls. Supernatants filtered during the first 96 hours of HHV-6A replication without visible cytopathic effect augmented HIV-1 syncytium formation by tenfold, reverse transcriptase activity by threefold, p24 antigen production by 6-fold. Filtered supernatants obtained at onset of HHV-6A cytopathic effect did not modify HIV-1 replication. HSB-2 cultures produced no IL-2, and IFN-gamma induced by endotoxin diminished HIV-1 replication. HHV-6A delayed IFN-gamma release. An increase in the tumour necrosis factor activity upon the effect of HHV-6A and endotoxin was not parallel to HIV-1 activation. The putative mediator, different from those above which characterisation is in progress, might transmit similar transactivating effects between immune cells of lymph nodes and circulation
Human Herpesvirus 6 variant A enanches in vitro HIV-1 replication by soluble mediators, which effect is diminisched by endotoxin
CECCHERINI-NELLI, LUCA;
1999-01-01
Abstract
Simultaneous HHV-6A infection can activate HIV-1 latency and promote AIDS progression, but in this process the effects of HHV-6A induced soluble mediators on HIV-1 have not been studied yet. Recently, supernatants of HSB-2 cultures infected with HHV-6A and/or treated with endotoxin have been filtered virus free at time intervals until the cytopathic effect developed. Biological activity of some cytokines which might participate in HIV-1 activation was quantitated. Filtered supernatants were mixed into CEM-ss cultures, which had been HIV-1 infected at 1:1 cell:virus ratio, subsequently HIV-1 replication was quantitated and compared to controls. Supernatants filtered during the first 96 hours of HHV-6A replication without visible cytopathic effect augmented HIV-1 syncytium formation by tenfold, reverse transcriptase activity by threefold, p24 antigen production by 6-fold. Filtered supernatants obtained at onset of HHV-6A cytopathic effect did not modify HIV-1 replication. HSB-2 cultures produced no IL-2, and IFN-gamma induced by endotoxin diminished HIV-1 replication. HHV-6A delayed IFN-gamma release. An increase in the tumour necrosis factor activity upon the effect of HHV-6A and endotoxin was not parallel to HIV-1 activation. The putative mediator, different from those above which characterisation is in progress, might transmit similar transactivating effects between immune cells of lymph nodes and circulationI documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
