Induction of B cell tolerance or activation was analyzed with vesicular stomatitis virus (VSV) glycoprotein (G) expressed as a neo-self Ag, A membrane form of VSV-C expressed in all tissues, including the bone marrow, induced unresponsiveness at both the Th and B cell level, whereas a soluble form of VSV-C expressed peripherally in liver and kidney did not tolerize B cells and only reversibly anergized Th cells, Interestingly, a similar correlation was found for activation of mature lymphocytes. When mature normal spleen cells were transferred into the two transgenic mouse lines, the membrane form of VSV-G was strongly immunogenic for both Th and B cells, and high VSV-G-specific IgG Ab titers were induced in these transgenic mice, In contrast, spleen cells transferred into mice expressing the soluble form of VSV-C were not activated, and no VSV-C specific Abs were induced, These results indicate that highly immunogenic Ags are strongly tolerogenic for both immature B and T cells.
Correlation of tolerogenicity of a viral antigen with its immunogenicity. (vol 158, pg 5106, 1997)
FREER, GIULIA;
1999-01-01
Abstract
Induction of B cell tolerance or activation was analyzed with vesicular stomatitis virus (VSV) glycoprotein (G) expressed as a neo-self Ag, A membrane form of VSV-C expressed in all tissues, including the bone marrow, induced unresponsiveness at both the Th and B cell level, whereas a soluble form of VSV-C expressed peripherally in liver and kidney did not tolerize B cells and only reversibly anergized Th cells, Interestingly, a similar correlation was found for activation of mature lymphocytes. When mature normal spleen cells were transferred into the two transgenic mouse lines, the membrane form of VSV-G was strongly immunogenic for both Th and B cells, and high VSV-G-specific IgG Ab titers were induced in these transgenic mice, In contrast, spleen cells transferred into mice expressing the soluble form of VSV-C were not activated, and no VSV-C specific Abs were induced, These results indicate that highly immunogenic Ags are strongly tolerogenic for both immature B and T cells.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
