Results generated by the immunohistochemical staining with PC10, a new monoclonal antibody recognizing PCNA (a nuclear protein associated with cell proliferation) in formalin-fixed and paraffin-embedded tissue were compared with those of Ki-67 labeling and DNA flow cytometry in 4 7 consecutive non-small cell lung cancer (NSCLC). PCNA reactivity was observed in all samples and confined to the nuclei of cancer cells. Its frequency ranged from 0 to 80% (37.7 +/- 23.6) and larger sized, early-staged and DNA aneuploid tumors expressed a significant higher number of PCNA-reactive cells. The PCNA and Ki-67 labeling rates were closely correlated (r = 0.383, P = 0. 009). By flow cytometry, we observed a good correlation among PCNA labeling and S-phase fraction (r = 0. 422, P = .0093) and G1 phase (r = 0.303, P = .051) of the cell cycle. Results indicate that PCNA labeling with PC10 is a simple method for assessing the proliferative activity in formalin-fixed, paraffin-embedded tissue of NSCLC and correlates well with Ki-67 labeling and S-phase fraction of the cell cycle.
|Autori:||FONTANINI G; PINGITORE R; BIGINI D; VIGNATI S; PEPE S; RUGGIERO A; MACCHIARINI P|
|Titolo:||GROWTH FRACTION IN NONSMALL CELL LUNG-CANCER ESTIMATED BY PROLIFERATING CELL NUCLEAR ANTIGEN AND COMPARISON WITH KI-67 LABELING AND DNA FLOW-CYTOMETRY DATA|
|Anno del prodotto:||1992|
|Appare nelle tipologie:||1.1 Articolo in rivista|