Purpose. Terbinafine is an allylamine derivative: it is the first antifungal agent with a primarily fungicidal action against dermatophytes. its good efficacy in treating dermatophythosis has already been demonstrated both in humans and animals. In this study, the authors checked two type of inocula, non sporulating and sporulating mycelia of Microsporum canis, to evaluate the in vitro activity of terbinafine using a microdilution test. Materials and methods. Twenty-nine unsporulated isolates and 18 sporulated samples of M canis were tested to terbinafine. All the isolates were obtained from the coats of cats by brushing technique and seeded onto Mycobiotic agar. The strains were successively seeded into Tryptic Soy Broth (TSB) or onto Potato Carrot Agar (PCA) to obtain the inocula to assay the susceptibility of both mycelium or macroconidia. The assays were carried out by microdilution. All tests were performed in quadruplicate. Results. All samples of unsporulated mycelia grew at concentrations of 0.005 mug/ml, 58 samples grew at concentrations of 0.025 mug/ml, 18 at 0.05 mug/ml. Growth at 0.5 mug/ml and higher concentrations was not observed. All samples of sporulating mycelia grew at 0.005 mug/ml, 24 grew at concentrations of 0.025 mug/ml, 20 at 0.05 mug/ml. No sample grew at concentrations of 0.5 mug/ml. When testing mycelia, a DE50 of 0.03 mug/ml has been recorded, while 0.02 mug/ml was the DE50 for the macroconidia. Conclusions. Basing upon the statistical analysis of the data, no difference between the behavior of the two different types of inocula towards terbinafine was observed. The strong fungicidal character of terbinafine against ill. canis has also been evidenced.
|Autori:||Nardoni S; Millanta F; Mancianti F|
|Titolo:||In vitro sensitivity of two different inocula of Microsporum canis versus terbinafine|
|Anno del prodotto:||2000|
|Appare nelle tipologie:||1.1 Articolo in rivista|