We measured three renal tubular brush-border enzymes (lactate dehydrogenase, LDH, EC 22.214.171.124; gamma-glutamyltransferase, GGT, EC 126.96.36.199; and alkaline phosphatase, AP, EC 188.8.131.52) in morning urine samples from 48 healthy subjects to check whether different storage times and temperatures could modify enzyme concentrations. Short-term (24 h) storage time at room temperature or 4 degree C does not affect urinary enzyme activity. A few days of freezing, at -20 or -70 degrees C, dramatically lowers LDH and AP values; GGT is partially preserved only at -70 degrees C, if the sample has been previously centrifuged. Urinary enzymes investigated in this study are extremely labile at low temperatures.
|Autori:||Matteucci E; Gregori G; Pellegrini L; Navalesi R; Giampietro O|
|Titolo:||Effects of storage time and temperature on urinary enzymes.|
|Anno del prodotto:||1991|
|Appare nelle tipologie:||1.1 Articolo in rivista|