Within the studies on the phenomenon of alkali-induced hair immunization, it has been observed that the hair immunization process arising from the action of dibasic alkalis takes place differently than immunization using monobasic alkalis. On the basis of these observations, our work investigated the influence of mono and divalent cations on the hair immunization mechanisms. In our experimental design, fresh salted bovine hides were exposed to mono and divalent alkalis and salts at different concentrations for various time periods. After treatment, the hides were exposed to reducing agents in order to verify the effectiveness of each cation in promoting immunization. We observed that immunization occurs with dibasic cations, but that both a minimum exposure time and a proper alkalinity are required. The appearance of inter and intra polypeptide chain links was investigated by comparing non-immunized and immunized hair using MIR and FIR spectroscopy, ICP chemical analysis and DSC. The chemical and the instrumental analyses indicate that the exposure of hair to divalent cations in alkaline medium, unlike the monovalent cations, induces the formation of cross-links in the keratin structure and promotes immunization. These investigations contribute to confirmation that the immunization process occurs only in the presence of divalent cations. Research work is in progress in order to further understand the nature and the mechanisms of these complex interactions

Studies on Mono and Divalent Cations Effects on Hair Immunization

CASTIELLO, DOMENICO;PUCCINI, MONICA;VITOLO, SANDRA
2007-01-01

Abstract

Within the studies on the phenomenon of alkali-induced hair immunization, it has been observed that the hair immunization process arising from the action of dibasic alkalis takes place differently than immunization using monobasic alkalis. On the basis of these observations, our work investigated the influence of mono and divalent cations on the hair immunization mechanisms. In our experimental design, fresh salted bovine hides were exposed to mono and divalent alkalis and salts at different concentrations for various time periods. After treatment, the hides were exposed to reducing agents in order to verify the effectiveness of each cation in promoting immunization. We observed that immunization occurs with dibasic cations, but that both a minimum exposure time and a proper alkalinity are required. The appearance of inter and intra polypeptide chain links was investigated by comparing non-immunized and immunized hair using MIR and FIR spectroscopy, ICP chemical analysis and DSC. The chemical and the instrumental analyses indicate that the exposure of hair to divalent cations in alkaline medium, unlike the monovalent cations, induces the formation of cross-links in the keratin structure and promotes immunization. These investigations contribute to confirmation that the immunization process occurs only in the presence of divalent cations. Research work is in progress in order to further understand the nature and the mechanisms of these complex interactions
2007
Castiello, Domenico; Puccini, Monica; Shelly, D.; Vitolo, Sandra
File in questo prodotto:
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11568/173460
 Attenzione

Attenzione! I dati visualizzati non sono stati sottoposti a validazione da parte dell'ateneo

Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 8
  • ???jsp.display-item.citation.isi??? 8
social impact