To investigate the relationship between oncogene activation and appearance of multidrug resistance (MDR) we transfected the human breast epithelial cell line MCF-10A, negative for the expression of the P-glycoprotein, with c-Ha-ras and/or c-erB-2 oncogenes. The appearance of the MDR phenotype was then studied by evaluating mdr-1 mRNA expression, the presence of P-glycoprotein on the cell membrane and the onset of doxorubicin resistance, together with the effect of the reversing agent verapamil. We found that only MCF-10A transfected with both c-Ha-ras and c-cerB-2 oncogenes acquired the MDR phenotype. (C) 1994 Wiley-Liss, Inc.
INDUCTION OF MULTIDRUG-RESISTANCE (MDR) BY TRANSFECTION OF MCF-10A CELL-LINE WITH C-HA-RAS AND C-ERBB-2 ONCOGENES
SABBATINI, ANTONIETTA RAFFAELLA MARIA;BASOLO, FULVIO;MATTII, LETIZIA;PETRINI, MARIO
1994-01-01
Abstract
To investigate the relationship between oncogene activation and appearance of multidrug resistance (MDR) we transfected the human breast epithelial cell line MCF-10A, negative for the expression of the P-glycoprotein, with c-Ha-ras and/or c-erB-2 oncogenes. The appearance of the MDR phenotype was then studied by evaluating mdr-1 mRNA expression, the presence of P-glycoprotein on the cell membrane and the onset of doxorubicin resistance, together with the effect of the reversing agent verapamil. We found that only MCF-10A transfected with both c-Ha-ras and c-cerB-2 oncogenes acquired the MDR phenotype. (C) 1994 Wiley-Liss, Inc.File in questo prodotto:
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