Twenty floral smelling tetrahydropyranyl and tetrahydrofuranyl ethers, and 12 additional compounds with different odours were used in ligand-binding experiments with purified 19 kDa bovine OBP and 22 kDa porcine OBP. Most of the odorants examined were found to be good ligands for both proteins, with dissociation constants in the micromolar range; the data confirm the broad binding specificity of OBPs. Significant differences, however, measured with some odorants, indicate the possibility of using OBPs, purified from several animal species, in biosensors for odour discrimination.

BINDING OF SELECTED ODORANTS TO BOVINE AND PORCINE ODORANT-BINDING PROTEINS

DAL MONTE, MASSIMO;PELOSI, PAOLO
1993

Abstract

Twenty floral smelling tetrahydropyranyl and tetrahydrofuranyl ethers, and 12 additional compounds with different odours were used in ligand-binding experiments with purified 19 kDa bovine OBP and 22 kDa porcine OBP. Most of the odorants examined were found to be good ligands for both proteins, with dissociation constants in the micromolar range; the data confirm the broad binding specificity of OBPs. Significant differences, however, measured with some odorants, indicate the possibility of using OBPs, purified from several animal species, in biosensors for odour discrimination.
DAL MONTE, Massimo; Centini, M.; Anselmi, C.; Pelosi, Paolo
File in questo prodotto:
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11568/175208
 Attenzione

Attenzione! I dati visualizzati non sono stati sottoposti a validazione da parte dell'ateneo

Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 40
  • ???jsp.display-item.citation.isi??? 38
social impact