Successful amplification of the whole intergenic spacer region of the nuclear ribosomal repeat (IGS) in Pyrenophora graminea was obtained with a PCR-based assay. Single amplification products showed length differences. Depending on the length of the IGS-PCR product, ca. 3.8 or 4.4 kb, two groups of isolates could be identified. The RFLP patterns of isolates obtained with the 6-base cutting enzymes ApaI, Bg/II, DraI, EcoRV, HindIII and SacI were similar within each group and different between the two groups. Restriction patterns of IGS-PCR products digested with the 4-base cutting enzyme AluI were polymorphic among isolates in spite of their IGS-PCR product length. In order to characterize the long and short IGS-PCR products the restriction map is shown. The long product shows an additional HindIII site and a Bg/II site that is lacking in the short product. However, the latter shows a SacI site that is not present in the long IGS-PCR product. Therefore: the described PCR-RFLP analysis of the IGS appears to be a useful tool to resolve genetic variation between P. graminea isolates. (C) 1998 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.
|Autori:||PECCHIA S; MERCATELLI E.; VANNACCI G.|
|Titolo:||PCR amplification and characterization of the intergenic spacer region of the ribosomal DNA in Pyrenophora graminea|
|Anno del prodotto:||1998|
|Digital Object Identifier (DOI):||10.1111/j.1574-6968.1998.tb13178.x|
|Appare nelle tipologie:||1.1 Articolo in rivista|