Polyacrylamide gel electrophoresis of water-soluble proteins from sunflower (Helianthus annuus L.) cotyledons, followed by specific staining for superoxide dismutase activity, discriminated, according to their electrophoretic mobility, two distinct achromatic bands for Cu/Zn superoxide dismutase. Zymograms of proteins from isolated chloroplasts showed that the chloroplast-located Cu/Zn superoxide dismutase (Cu/ZnSODchl) migrated faster in the SOD activity-stained gels. An electrophoretic variant pattern, whose mobility is lower than the control pattern, was identified in the ABA-deficient mutant w-1. The variant is coded by a nuclear gene with two codominant alleles.
Genetic analysis of an electrophoretic variant for the chloroplast-associated form of Cu/Zn superoxide dismutase in sunflower (Helianthus annuus L.)
FAMBRINI, MARCO;CAVALLINI, ANDREA;PUGLIESI, CLAUDIO
1997-01-01
Abstract
Polyacrylamide gel electrophoresis of water-soluble proteins from sunflower (Helianthus annuus L.) cotyledons, followed by specific staining for superoxide dismutase activity, discriminated, according to their electrophoretic mobility, two distinct achromatic bands for Cu/Zn superoxide dismutase. Zymograms of proteins from isolated chloroplasts showed that the chloroplast-located Cu/Zn superoxide dismutase (Cu/ZnSODchl) migrated faster in the SOD activity-stained gels. An electrophoretic variant pattern, whose mobility is lower than the control pattern, was identified in the ABA-deficient mutant w-1. The variant is coded by a nuclear gene with two codominant alleles.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
