OBJECTIVE: To study the specificity of antibodies reactive with renal antigens in mixed cryoglobulinemia. METHODS: Sera from mixed cryoglobulinemia (MC) patients were tested on human kidney extracts by immunoblot. A partially purified renal antigen was subjected to N-terminal sequencing. RESULTS: Antibodies reactive with a renal antigen of 48 kD were detected in 7 out of 11 patients with MC and renal involvement. N-terminal sequencing of this antigen showed that it was identical with alpha-enolase. This result was confirmed by the reactivity of the renal antigen with a rabbit anti-serum specific for alpha-enolase.CONCLUSIONS: The results indicate that antibodies specific for alpha-enolase are frequently produced by mixed cryoglobulinemia patients with renal involvement.
Alpha-enolase is a renal specific antigen associated with kidney involvement in mixed cryoglobulinemia
MARCHINI, BARBARA;MOSCATO, STEFANIA;PRATESI, FEDERICO;MIGLIORINI, PAOLA
1997-01-01
Abstract
OBJECTIVE: To study the specificity of antibodies reactive with renal antigens in mixed cryoglobulinemia. METHODS: Sera from mixed cryoglobulinemia (MC) patients were tested on human kidney extracts by immunoblot. A partially purified renal antigen was subjected to N-terminal sequencing. RESULTS: Antibodies reactive with a renal antigen of 48 kD were detected in 7 out of 11 patients with MC and renal involvement. N-terminal sequencing of this antigen showed that it was identical with alpha-enolase. This result was confirmed by the reactivity of the renal antigen with a rabbit anti-serum specific for alpha-enolase.CONCLUSIONS: The results indicate that antibodies specific for alpha-enolase are frequently produced by mixed cryoglobulinemia patients with renal involvement.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
