A reliable and low-cost method that enables rapid screening of the activity exerted by new antimicrobial agents on intracellularly growing Mycobacterium avium has been developed. To this aim, a recombinant (lacZ) strain of M. avium expressing the Escherichia call beta -galactosidase gene was used to evaluate, in murine macrophages, the susceptibility of M. avium to common antimycobacterial agents. beta -Galactosidase levels, measured in the presence of each of the antibiotics tested, were closely correlated with the number of CFU recovered from the M. avium lacZ strain-infected macrophages.
|Autori:||MAISETTA G; BATONI G; PARDINI M; BOSCHI A; BOTTAI D; ESIN S; CAMPA M; SENESI S|
|Titolo:||Use of a recombinant strain of Mycobacterium avium expressing beta-galactosidase to evaluate the activities of antimycobacterial agents inside macrophages|
|Anno del prodotto:||2001|
|Digital Object Identifier (DOI):||10.1128/AAC.45.1.356-358.2001|
|Appare nelle tipologie:||1.1 Articolo in rivista|