A total of 62 canine melanocytic tumours (10 melanocytomas and 52 primary malignant melanomas) were investigated to compare the accuracy of prognosis provided by MIB-1 proliferation index (MIB-1-PI) with classical histological criteria and location. MIB-1-PI was assessed by means of quantitative image analysis of sections immunostained with MIB-1 monoclonal antibody. Tumour location, histological cell type, stromal or lymphatic vessel invasion, maximum tumour thickness, and presence of inflammation or necrosis were recorded for each case. Thirty-eight dogs were submitted to a 1-year follow-up and the clinical outcome of the disease determined. MIB-1-PI in melanocytomas differed significantly from that detected in primary malignant melanomas (P = 0.0001). A significant difference in MIB-1-PI was revealed between oral and cutaneous malignant melanomas (P = 0.015), and between presence and absence of lymphatic vessel invasion (P = 0.05). MIB-1-PI was not correlated with the other parameters. In univariate analysis, only turnout location (oral vs cutaneous), presence of lymphatic vessel invasion, and MIB-1-PI were associated with decreased overall survival (P = 0.0001, P = 0.0144, and P = 0.0489, respectively). In conclusion, the results of our study confirm that the assessment of the MIB-1-PI may be of additional prognostic value for dogs with primary malignant melanomas. (C) 2002 Elsevier Science Ltd. All rights reserved.
|Autori:||MILLANTA F; FRATINI F; CORAZZA M; CASTAGNARO M; ZAPPULLI V; POLI A|
|Titolo:||Proliferation activity in oral and cutaneous canine melanocytic tumours: correlation with histological parameters, location, and clinical behaviour|
|Anno del prodotto:||2002|
|Digital Object Identifier (DOI):||10.1016/S0034-5288(02)00041-3|
|Appare nelle tipologie:||1.1 Articolo in rivista|