Two soluble proteins were isolated as major secretory products of horse sweat and of the parotid gland and characterized for structural and functional properties. The first protein, lipocalin allergen EquC1, was characterized for its glycosylation sites and bound glycosidic moieties. Only one (Asn53) of the two putative glycosylation sites within the sequence was post-translationally modified; a different glycosylation pattern was determined with respect to data previously reported. When purified from horse sweat, this protein contained oleamide and other organic molecules as natural ligands. Ligand binding experiments indicated good protein selectivity toward volatile compounds having a straight chain structure of 9-11 carbon atoms, suggesting a role of this lipocalin in chemical communication. The second protein, here reported for the first time in the horse, belongs to the group of parotid secretory proteins, part of a large superfamily of binding proteins whose function in most cases is still unclear. This protein was sequenced and characterized for its post-translational modifications. Of the three cysteine residues present, two were involved in a disulfide bridge (Cys155-Cys198). A model, built up on the basis of similar proteins, indicated a general fold characterized by the presence of a long hydrophobic barrel. Binding experiments performed with a number of different organic compounds failed to identify ligands for this protein with a well-defined physiological role.
Secretory proteins as potential semiochemical carriers in the horse / D'Innocenzo, B; Salzano, Am; D'Ambrosio, C; Gazzano, Angelo; Niccolini, Alberto; Sorce, Carlo; Dani, Fr; Scaloni, A; Pelosi, Paolo. - In: BIOCHEMISTRY. - ISSN 0006-2960. - ELETTRONICO. - 45(2006), pp. 13418-13428.
|Autori:||D'Innocenzo, B; Salzano, Am; D'Ambrosio, C; Gazzano, Angelo; Niccolini, Alberto; Sorce, Carlo; Dani, Fr; Scaloni, A; Pelosi, Paolo|
|Titolo:||Secretory proteins as potential semiochemical carriers in the horse|
|Anno del prodotto:||2006|
|Digital Object Identifier (DOI):||10.1021/bi061409p|
|Appare nelle tipologie:||1.1 Articolo in rivista|